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NCEMS simplified sequencing pipelines

Radically slimmed descendants of the ENCODE processing pipelines (with the custom allo multimapper-reallocation step), built for efficient peak calling with IDR.

  • chip-seq-allo-pipeline/ — TF/histone ChIP-seq: fastq → trim → bowtie2 → filter → allo → MACS2 (on BAM) → IDR. Controls are pre-built into a common BAM library and sourced lazily. See its README.
  • atac-seq-allo-pipeline/ — ATAC-seq (control-free): fastq → trim → bowtie2 → filter → allo (--atac) → ATAC MACS2 cut-site model (on BAM) → IDR. See its README.

Each pipeline is a self-contained Caper/WDL workflow with its own conda envs, cluster submit scripts, and input-JSON generator.